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HelpTest Code TPPA Syphilis Antibody, Treponema pallidum Particle Agglutination, Serum
Ordering Guidance
This assay is recommended by the Centers for Disease Control and Prevention for sera testing positive by a screening treponemal assay and negative by rapid plasma reagin (RPR). The results of this assay assist in determining whether the results of a screening treponemal test are truly or falsely positive.
For prenatal syphilis screening, the syphilis IgG test (SYPH1 / Syphilis IgG with Reflex, Enzyme Immunoassay, Serum) is recommended. Testing for IgM-class antibodies to T pallidum should not be performed during routine pregnancy screening.
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
CMH COLLECTION: 2 mL in red gel vacutainer
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 14 days |
Testing Algorithm
For more information see Syphilis Serology Algorithm.
Useful For
An aid to resolve discrepant results between screening treponemal and non-treponemal assays
This test is not recommended for general screening purposes for syphilis.
This test should not be used to evaluate response to therapy.
This test is not intended for medical-legal use.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Day(s) Performed
Monday through Friday
Report Available
Same day/1 to 4 daysReporting Name
Syphilis Ab by TP-PA, SReference Values
Negative
Method Name
Particle Agglutination
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86780