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HelpTest Code TGRP Testosterone, Total and Free, Serum
Ordering Guidance
This is a second-level test for suspected increases or decreases in physiologically active testosterone. The preferred test for assessment of active testosterone is TTBS / Testosterone, Total and Bioavailable, Serum.
Necessary Information
Patient's age and sex are required.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 2.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial
CMH Collection: 4 mL in Red vacutainer
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Refrigerated (preferred) | 14 days | |
| Frozen | 60 days |
Useful For
Alternative, second-level test for suspected increases or decreases in physiologically active testosterone:
-Assessment of androgen status in cases with suspected or known sex hormone-binding globulin-binding abnormalities
-Assessment of functional circulating testosterone in early pubertal boys and older men
-Assessment of functional circulating testosterone in women with symptoms or signs of hyperandrogenism but normal total testosterone levels
-Monitoring of testosterone therapy or antiandrogen therapy in older men and in female patients
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Day(s) Performed
Monday through Saturday
Report Available
3 to 8 daysReporting Name
Testosterone, Total and Free, SReference Values
TESTOSTERONE, FREE
Males (adult):
20-<25 years: 5.25-20.7 ng/dL
25-<30 years: 5.05-19.8 ng/dL
30-<35 years: 4.85-19.0 ng/dL
35-<40 years: 4.65-18.1 ng/dL
40-<45 years: 4.46-17.1 ng/dL
45-<50 years: 4.26-16.4 ng/dL
50-<55 years: 4.06-15.6 ng/dL
55-<60 years: 3.87-14.7 ng/dL
60-<65 years: 3.67-13.9 ng/dL
65-<70 years: 3.47-13.0 ng/dL
70-<75 years: 3.28-12.2 ng/dL
75-<80 years: 3.08-11.3 ng/dL
80-<85 years: 2.88-10.5 ng/dL
85-<90 years: 2.69-9.61 ng/dL
90-<95 years: 2.49-8.76 ng/dL
95-100+ years: 2.29-7.91 ng/dL
Males (children):
<1 year: Term infants
1-15 days: 0.20-3.10 ng/dL*
16 days-1 year: Values decrease gradually from newborn (0.20-3.10 ng/dL) to prepubertal levels
*Forest MG, Cathiard AM, Bertrand JA. Total and unbound testosterone levels in the newborn and in normal and hypogonadal children: use of a sensitive radioimmunoassay for testosterone. J Clin Endocrinol Metab. 1973;36(6):1132-1142
1-8 years: <0.13 ng/dL
9 years: <0.13-0.45 ng/dL
10 years: <0.13-1.26 ng/dL
11 years: <0.13-5.52 ng/dL
12 years: <0.13-9.28 ng/dL
13 years: <0.13-12.6 ng/dL
14 years: 0.48-15.3 ng/dL
15 years: 1.62-17.7 ng/dL
16 years: 2.93-19.5 ng/dL
17 years: 4.28-20.9 ng/dL
18 years: 5.40-21.8 ng/dL
19 years: 5.36-21.2 ng/dL
Females (adult):
20-<25 years: <0.13-1.08 ng/dL
25-<30 years: <0.13-1.06 ng/dL
30-<35 years: <0.13-1.03 ng/dL
35-<40 years: <0.13-1.00 ng/dL
40-<45 years: <0.13-0.98 ng/dL
45-<50 years: <0.13-0.95 ng/dL
50-<55 years: <0.13-0.92 ng/dL
55-<60 years: <0.13-0.90 ng/dL
60-<65 years: <0.13-0.87 ng/dL
65-<70 years: <0.13-0.84 ng/dL
70-<75 years: <0.13-0.82 ng/dL
75-<80 years: <0.13-0.79 ng/dL
80-<85 years: <0.13-0.76 ng/dL
85-<90 years: <0.13-0.73 ng/dL
90-<95 years: <0.13-0.71 ng/dL
95-100+ years: <0.13-0.68 ng/dL
Females (children):
<1 year: Term infants
1-15 days: <0.13-0.25 ng/dL*
16 days-1 year: Values decrease gradually from newborn (<0.13-0.25 ng/dL) to prepubertal levels
*Forest MG, Cathiard AM, Bertrand JA. Total and unbound testosterone levels in the newborn and in normal and hypogonadal children: use of a sensitive radioimmunoassay for testosterone. J Clin Endocrinol Metab. 1973;36(6):1132-1142
1-4 years: <0.13 ng/dL
5 years: <0.13 ng/dL
6 years: <0.14 ng/dL
7 years: <0.13-0.23 ng/dL
8 years: <0.13-0.34 ng/dL
9 years: <0.13-0.46 ng/dL
10 years: <0.13-0.59 ng/dL
11 years: <0.13-0.72 ng/dL
12 years: <0.13-0.84 ng/dL
13 years: <0.13-0.96 ng/dL
14 years: <0.13-1.06 ng/dL
15-18 years: <0.13-1.09 ng/dL
19 years: <0.13-1.08 ng/dL
TESTOSTERONE, TOTAL
Males
0-5 months: 75-400 ng/dL
6 months-9 years: <7-20 ng/dL
10-11 years: <7-130 ng/dL
12-13 years: <7-800 ng/dL
14 years: <7-1,200 ng/dL
15-16 years: 100-1,200 ng/dL
17-18 years: 300-1,200 ng/dL
≥19 years: 240-950 ng/dL
Tanner Stages**
I (prepubertal): <7-20
II: 8-66
III: 26-800
IV: 85-1,200
V (young adult): 300-950
Females
0-5 months: 20-80 ng/dL
6 months-9 years: <7-20 ng/dL
10-11 years: <7-44 ng/dL
12-16 years: <7-75 ng/dL
17-18 years: 20-75 ng/dL
≥19 years: 8-60 ng/dL
Tanner Stages**
I (prepubertal): <7-20
II: <7-47
III: 17-75
IV: 20-75
V (young adult): 12-60
**Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (±2) years and for girls at a median age of 10.5 (±2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. For boys, there is no definite proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (young adult) should be reached by age 18.
Method Name
FRTST: Equilibrium Dialysis/Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
TTST: Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84402
84403
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| FRTST | Testosterone, Free, S | No | Yes |
| TTST | Testosterone, Total, S | Yes | Yes |