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STR - Chimerism Analysis , cells sorted for CD3, CD19, CD33, CD56

Collection Requirements

Source: post-transplant blood or bone marrow
Container: EDTA lavender
Volume: 8-10 mL in vacutainer

Special Instructions

  • Transport at room temperature; avoid fluctuating temperatures (hot or cold)
  • Send immediately; delays impact specimen integrity
  • Use the Oncology Cytogenetics Requisition Neoplastic

Turn Around Time

5 days

Availability

Routine

Lab Processing Instructions

Specimen: whole blood or bone marrow
Room temperature

Performing Laboratory

Genetics

Adele Hall Lab Section

Cytogenetics

Instrumentation/Methodology

STR Chimerism Testing

Additional Information

PCR amplification of 22 Short Tandem Repeat (STR) Loci (Penta E, D18S51, D21S11, THO1, D3S1358, FGA, TPOX, D8S1179, vWA, Penta D, CSF1P0, D16S539, D7S820, D13S317, D5S818, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433, and D22S1045), as well as Amelogenin and DYS391 for gender confirmation, is performed utilizing fluorescently labeled markers. Fragment analysis and host/donor DNA quantitation is performed with an ABI-3500 DNA sequencer/fragment analyzer. Analytical sensitivity to detect chimerism is 1-5%. The reportable range is 0-100%. Traces of chimerism may be reported.

Reference Ranges

See interpretive report

CPT

81268 – for each cell sort