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Chromosome Analysis, amniotic fluid

Collection Restrictions

Gestational age should be at least 14 weeks, but preferably 15-20 weeks

Collection Requirements

Source: amniotic fluid
Container: sterile clear centrifuge tubes
Volume: breakdown based on ordered tests

  • Chromosome analysis: 15-20 mL
  • FISH analysis: 4 mL
  • Alpha-Fetoprotein: 1 mL
  • Toxoplasmosis, CMV, HSV, etc: 1 mL for each test
  • Microarray or NGS:
    • 15 mL if ordered with chromosome analysis
    • 25 mL if ordered without chromosome analysis
    • AND 2-3 mL maternal blood in EDTA lavender vacutainer

Special Instructions

  • Transport at room temperature; avoid fluctuating temperatures (hot or cold)
  • Cap tubes tightly
  • Specimen must be processed within 24 hours of collection
  • Contact Genetics for collection materials
  • Use the Prenatal and Pregnancy Loss Cytogenetics Requisition

Turn Around Time

14 days

Availability

Routine

Lab Processing Instructions

Specimen: amniotic fluid
Room temperature

Performing Laboratory

Genetics

Adele Hall Lab Section

Cytogenetics

Instrumentation/Methodology

Primarily In situ cell culture. The cells are processed by exposure to colcemid and hypotonic solution, then fixed. Slides are prepared and metaphase cells are GTG banded. Chromosomes analysis and image captured are performed on a computerized imaging system.

Additional Information

Indications for testing: advanced maternal age (>35 years of age); Previous pregnancy with a chromosome abnormality; Parent carrying a known translocation; Abnormal ultrasound findings; Abnormal NIPS

Reference Ranges

See interpretive report

CPT

88267, 88280, 88235