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B and T Cell Rearrangement

Collection Requirements

Source: blood or bone marrow
Container: EDTA lavender
Volume:

  • Preferred: 3 mL in vacutainer
  • Minimum: 1 mL in vacutainer

Source: tumor, fresh frozen
Container: sterile cup
Volume: 20 mg


Source: tissue, formalin fixed paraffin embedded

  • Slides: 2 unstained sections (4 micron thick sections, sequential cut)
  • Scrolls: 5 scrolls (10 microns think in 1.5 mL tube)

Special Instructions

Specify newly diagnosed disease or minimum residual disease; provide tumor % for FFPE and fresh frozen solid tissue

Turn Around Time

7 days

Availability

Routine

Lab Processing Instructions

Specimen: whole blood or bone marrow Room temperature or refrigerate Specimen: tumor, fresh frozen Freeze Specimen: Formalin fixed parafin embbeded Room temperature

Performing Laboratory

Adele Hall

Adele Hall Lab Section

Molecular Genetics

Instrumentation/Methodology

PCR/ fragment analysis

Additional Information

IGH (B-cell) and TCR (T-cell receptor) gamma gene rearrangements are used a tool for assisting in the diagnosis of suspected lymphoproliferative disorders. Detection of a monoclonal IGH or TCR gamma gene rearrangement is suggestive (but, not solely diagnostic) of a neoplastic process whereas polyclonality usually indicates a reactive proliferation.

Reference Ranges

See interpretative report

CPT

B-cell 81261 +
T-cell 81342