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HelpTest Code IFBA Intrinsic Factor Blocking Antibody, Serum
Ordering Guidance
For a comprehensive workup of patients with suspected pernicious anemia, order ACASM / Pernicious Anemia Cascade, Serum, which initiates testing with measurement of vitamin B12. Depending of the vitamin B12 concentration, testing for intrinsic factor blocking antibody, gastrin, and methylmalonic acid may be added.
Specimen Required
Patient Preparation:
1. Patient should be fasting for 8 hours.
2. This test should not be performed on patients who have received a vitamin B12 injection or radiolabeled vitamin B12 injection within the previous 2 weeks.
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
CMH COLLECTION: 1mL blood in a vacutainer Preferred: Red gel Acceptable: Red
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Testing Algorithm
For more information, see Vitamin B12 Deficiency Evaluation.
Useful For
Confirming the diagnosis of pernicious anemia
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
Day(s) Performed
Monday through Friday
Report Available
1 day to 3 daysReporting Name
Intrinsic Factor Blocking Ab, SReference Values
Negative
Method Name
Immunoenzymatic Assay
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86340