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Flow B Cell Naive/Memory

Collection Restrictions

Collections planned for Friday after 3:00 p.m. - Monday 4:00 a.m., or on holidays
that will require immediate testing: Call Flow Cytometry on Friday before 3:00 p.m.

 

Collection Requirements

Source: blood
Container: EDTA lavender
Volume:

  • Preferred: 1 mL in vacutainer 
  • Acceptable: microtainer filled to the top line

Special Instructions

CBC with differential request must accompany Flow Cytometry test requests in order to report results

Turn Around Time

24-72 hours
Collections done Friday - Sunday will have a longer TAT

Availability

Routine

Includes

Antibody staining for cell surface markers CD19, CD45, CD27, and IgD

Lab Processing Instructions

Specimen: whole blood
Room temperature

Performing Laboratory

Adele Hall

Adele Hall Lab Section

Flow Cytometry

Instrumentation/Methodology

Flow Cytometry

Additional Information

Useful for assessing patients that may have a common variable immunodeficiency disorder and to assay for reconstitution of the patient’s B-cell humoral immunity post bone marrow transplant

Reference Ranges

Age Absolute (cell/mcL) Percent
 B Cells (CD19+)  Naive B Cells (CD19+CD27-IgGD+)  Memory B Cells  Class Switched Memory B Cells Naive B Cells of Total B Cells Memory B Cells (CD19+CD27+IgD±) of Total B Cells Class Switched Memory B Cells (CD19+CD27+IgD-) of Total B
Birth - 1 Month 350-600 Not established yet See below See below See below See below See below
1-6 Months 500-1800 Not established yet See below See below See below See below See below
6 Months - 2 Years 400-1800 Not established yet See below See below See below See below See below
2-4 Years 200-1000 Not established yet See below See below See below See below See below
4-8 Years 150-800 Not established yet See below See below See below See below See below
8-12 Years 100-700 Not established yet See below See below See below See below See below
12-21 Years 75-600 Not established yet See below See below See below See below See below
>21 Years 50-550 Not established yet See below See below See below See below See below
Full Term Cord Blood 200-1900 Not established yet See below See below See below See below See below
0-6 Months See above See above 10-44 1-10 Not established yet 1-7 0-3
6 Months - 1 Year See above See above 16-72 2-16 Not established yet 3-10 1-4
1-2 Years See above See above 38-90 10-30 Not established yet 4-11 2-6
2-5 Years See above See above 50-110 15-35 Not established yet 7-28 5-13
5-12 Years See above See above 40-70 15-30 Not established yet 10-37 6-14
12-18 Years See above See above 35-65 10-30 Not established yet 12-45 5-14
>18 Years See above See above 30-60 20-40 Not established yet 14-40 7-14

 

CPT

86356 x3 
86355 (if applicable)