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Flow B Cell Naive/Memory

Collection Restrictions

Collections planned for Friday after 3:00 p.m. - Monday 4:00 a.m., or on holidays
that will require immediate testing: Call Flow Cytometry on Friday before 3:00 p.m.

 

Collection Requirements

Source: blood
Container: EDTA lavender
Volume:

  • Preferred: 1 mL in vacutainer 
  • Acceptable: microtainer filled to the top line

Special Instructions

CBC with differential request must accompany Flow Cytometry test requests in order to report results

Includes

Antibody staining for cell surface markers CD19, CD45, CD27, and IgD

Turn Around Time

24-72 hours
Collections done Friday - Sunday will have a longer TAT

Availability

Routine

Lab Processing Instructions

Specimen: whole blood
Room temperature

Performing Laboratory

Adele Hall

Adele Hall Lab Section

Flow Cytometry

Instrumentation/Methodology

Flow Cytometry

Reference Ranges

Age Absolute (cell/mcL) Percent
 B Cells (CD19+)  Naive B Cells (CD19+CD27-IgGD+)  Memory B Cells  Class Switched Memory B Cells Naive B Cells of Total B Cells Memory B Cells (CD19+CD27+IgD±) of Total B Cells Class Switched Memory B Cells (CD19+CD27+IgD-) of Total B
Birth - 1 Month 350-600 Not established yet See below See below See below See below See below
1-6 Months 500-1800 Not established yet See below See below See below See below See below
6 Months - 2 Years 400-1800 Not established yet See below See below See below See below See below
2-4 Years 200-1000 Not established yet See below See below See below See below See below
4-8 Years 150-800 Not established yet See below See below See below See below See below
8-12 Years 100-700 Not established yet See below See below See below See below See below
12-21 Years 75-600 Not established yet See below See below See below See below See below
>21 Years 50-550 Not established yet See below See below See below See below See below
Full Term Cord Blood 200-1900 Not established yet See below See below See below See below See below
0-6 Months See above See above 10-44 1-10 Not established yet 1-7 0-3
6 Months - 1 Year See above See above 16-72 2-16 Not established yet 3-10 1-4
1-2 Years See above See above 38-90 10-30 Not established yet 4-11 2-6
2-5 Years See above See above 50-110 15-35 Not established yet 7-28 5-13
5-12 Years See above See above 40-70 15-30 Not established yet 10-37 6-14
12-18 Years See above See above 35-65 10-30 Not established yet 12-45 5-14
>18 Years See above See above 30-60 20-40 Not established yet 14-40 7-14

 

CPT

86356 x3 
86355 (if applicable)

Additional Information

Useful for assessing patients that may have a common variable immunodeficiency disorder and to assay for reconstitution of the patient’s B-cell humoral immunity post bone marrow transplant