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HelpFlow T Cell Naive/Memory with Recent Thymic Emigrants
Collection Restrictions
Collections planned for Friday after 3:00 p.m. - Monday
4:00 a.m., or on holidays
that will require immediate testing: Call Flow Cytometry on Friday
before 3:00 p.m.
Collection Requirements
Source: blood
Container: EDTA lavender
Volume:
- Preferred: 1 mL in vacutainer
- Acceptable: microtainer filled to the top line
Special Instructions
CBC with differential request must accompany Flow Cytometry test requests in order to report results.
Recommend a standard lymphocyte immunophenotyping panel accompany this order: Flow T Cell Immune Deficiency, Flow TBNK Lymphocyte Enumeration or Extended Immune Deficiency Panel
Turn Around Time
24-72 hours
Collections done Friday -Sunday will have a longer TAT
Availability
Routine
Includes
Antibody staining for cell surface markers CD3, CD4, CD8, CD31, CD45, CD45RA, CD45RO, and CD62L
Lab Processing Instructions
Specimen: whole blood
Do not refrigerate
Performing Laboratory
Adele Hall
Adele Hall Lab Section
Flow Cytometry
Instrumentation/Methodology
Flow Cytometry
Additional Information
For characterization of thymic function in production of T cells by enumeration of T cell Naïve and Memory subsets in congenital immune deficiencies. Also useful for monitoring immune reconstitution following immune suppression, immunotoxic therapies, or BMT.
Reference Ranges
All values are Absolute (cells/mcL)
| Age | #CD4+ CD45RA+ CD31+ (CD4+RTE's) |
#CD4+ CD45RA+ CD62L+ (Naive T Cells) |
#CD4+ CD45RO+ (Total Memory T Cells) |
#CD8+ CD45RA+ CD62L+ (Naive T Cells) |
#CD8+ CD45RO+ (Total Memory T Cells) |
|---|---|---|---|---|---|
| 0-2 months | Not Established Yet | 1200-3600 | 60-900 | 380-1300 | 30-330 |
| 3-6 months | Not Established Yet | 1300-3600 | 120-630 | 450-1200 | 30-290 |
| 7-12 months | Not Established Yet | 1100-3600 | 160-800 | 330-1200 | 40-330 |
| 12 months - 2 years | Not Established Yet | 950-2800 | 210-850 | 400-1400 | 60-570 |
| 3-6 years | Not Established Yet | 420-1500 | 220-660 | 260-850 | 90-440 |
| 7-12 years | Not Established Yet | 310-1000 | 230-630 | 200-650 | 70-390 |
| 13-18 years | Not Established Yet | 210-750 | 240-700 | 170-560 | 60-310 |
| >18 years | Not Established Yet | 200-700 | 250-750 | 150-500 | 50-280 |
| Age |
#CD4+ CD45RA+ CD31+ (Recent Thymic Emigrants) |
|---|---|
| 1 week - 2 months | 1000-4900 |
| 2-5 months | 1400-5200 |
| 5-9 months | 800-6200 |
| 9-15 months | 900-5800 |
| 15-24 months | 170-7400 |
| 2-5 years | 190-2600 |
| 5-10 years | 200-1700 |
| 10-16 years | 150-1500 |
| >19 years | 51-609 |
CPT
86356 x7
86359