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HelpFlow T Cell Immune Deficiency Panel
Collection Restrictions
Collections planned for Friday after 3:00 p.m. - Monday 4:00 a.m., or on holidays
that will require immediate testing: Call Flow Cytometry on Friday before 3:00 p.m.
Collection Requirements
Source: blood
Container: EDTA lavender
Volume:
- Preferred: 1 mL in vacutainer
- Acceptable: microtainer filled to the top line
Other sources: Call Flow Cytometry
Special Instructions
CBC with differential request must accompany Flow Cytometry test requests in order to report results
Includes
Antibody staining for cell surface markers CD3, CD4, CD8, and CD45
Turn Around Time
24-72 hours
Collection done Friday - Sunday will have a longer TAT
Other sources: 48 hours
Availability
Routine
Lab Processing Instructions
Specimen: whole blood or bone marrow
Room temperature
Specimen(s): body fluids (cell suspensions) and tissue in RPMI media
Refrigerate
Performing Laboratory
Adele Hall
Adele Hall Lab Section
Flow Cytometry
Instrumentation/Methodology
Flow Cytometry
Reference Ranges
| Age | Total T Cells (CD3+) Absolute | T Helper Cells (CD3+CD4+) Absolute | T Cytotoxic Cells (CD3+CD8+) Absolute | Helper/Cytotoxic Ratio (CD4/CD8) |
|---|---|---|---|---|
| 0 Day - 1 Month | 1600-5500 | 1200-4200 | 400-2100 | 1.20-2.99 |
| 1-6 Months | 2000-6500 | 1500-5100 | 650-2500 | 1.20-2.99 |
| 6 Months - 2 Years | 1500-5600 | 1300-3600 | 550-2300 | 1.20-2.99 |
| 2-4 Years | 1600-4300 | 900-2900 | 600-1900 | 1.20-2.99 |
| 4-8 Years | 1100-3400 | 500-2100 | 400-1100 | 1.20-2.99 |
| 8-12 Years | 900-2900 | 450-1600 | 300-1000 | 1.20-2.99 |
| 12-21 Years | 900-2600 | 500-1700 | 250-1000 | 1.20-2.99 |
| >21 Years | 700-2800 | 300-1900 | 200-1000 | 1.20-2.99 |
| Full term cord blood | 1300-5100 | 900-3700 | 300-1600 | 1.20-2.99 |
CPT
86356 (may be added mutliple times as applicable)
86359
86360
Additional Information
This test is useful for the assessment of immune deficiencies and monitoring transplant rejection therapy