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HelpFlow Extended Immune Deficiency Panel (EIDP)
Collection Restrictions
Collections planned for Friday after 3:00 p.m. - Monday 4:00 a.m., or on holidays
that will require immediate testing: Call Flow Cytometry on Friday before 3:00 p.m.
Collection Requirements
Source: blood
Container: EDTA lavender
Volume:
- Preferred: 1 mL in vacutainer
- Acceptable: Microtainer filled to top line
Special Instructions
CBC with differential request must accompany Flow Cytometry test requests in order to report results
Includes
Antibody staining for cell surface markers CD2, CD3, CD4, CD8, CD19, CD45, CD16,56, CD25+/CD3+, HLA-DR+/CD3, and CD16,56+/CD3+
Turn Around Time
24-72 hours
Collections done Friday-Sunday will have a longer TAT
Availability
Routine
Lab Processing Instructions
Specimen: whole blood
Room temperature
Performing Laboratory
Adele Hall
Adele Hall Lab Section
Flow Cytometry
Instrumentation/Methodology
Flow Cytometry
Reference Ranges
| Age | Pan T Cells (CD2+) Absolute | Total T Cells (CD3+) Absolute | T Helper Cells (CD3+CD4+) Absolute | T Cytotoxic Cells (CD3+CD8+) Absolute | Helper/Cytotoxic Ratio (CD4/CD8) |
Total B Cells (CD19+) Absolute
|
Natural Killer Cels (CD16+CD56+CD3-) Absolute |
|---|---|---|---|---|---|---|---|
| 0 - 1 Month | 2500-5500 | 1600-5500 | 1200-4200 | 400-2100 | 1.20-2.99 | 350-600 | 80-340 |
| 1-6 Months | 3500-6500 | 2000-6500 | 1500-5100 | 650-2500 | 1.20-2.99 | 500-1800 | 80-340 |
| 6 Months - 2 Years | 3000-5600 | 1500-5600 | 1300-3600 | 550-2300 | 1.20-2.99 | 400-1800 | 80-340 |
| 2-4 Years | 2000-4500 | 1600-4300 | 900-2900 | 600-1900 | 1.20-2.99 | 200-1000 | 80-662 |
| 4-8 Years | 1200-4000 | 1100-3400 | 500-2100 | 400-1100 | 1.20-2.99 | 150-800 | 80-662 |
| 8-12 Years | 1100-3800 | 900-2900 | 450-1600 | 300-1000 | 1.20-2.99 | 100-700 | 80-662 |
| 12-21 Years | 1100-3200 | 900-2600 | 500-1700 | 250-1000 | 1.20-2.99 | 75-600 | 80-662 |
| >21 Years | 1000-3000 | 700-2800 | 300-1900 | 200-1000 | 1.20-2.99 | 50-550 | 80-662 |
| Full term cord blood | 1300-5100 | 900-3700 | 300-1600 | 1.20-2.99 | 200-1900 |
CPT
86355
86356 x5
86357
86359
86360
Additional Information
This test is useful for the assessment of immune deficiencies and post BMT monitoring