Flow DNA Ploidy and Cell Cycle Analysis
Collection Requirements
Source: blood or bone marrow
Container: EDTA lavender
Volume:
- Preferred: 1 mL in vacutainer
- Acceptable: microtainer filled to top line
Other sources: Call Flow Cytometry
Availability
Routine
Turn Around Time
3 days
Lab Processing Instructions
Specimen: whole blood or bone marrow
Room temperature
Specimen(s): body fluids (cell suspensions) and tissue in RPMI
media
Refrigerate
Performing Laboratory
Adele Hall
Adele Hall Lab Section
Flow Cytometry
Additional Information
This test is useful for the assessment of DNA ploidy and proliferative phase fractions of cell populations.
Instrumentation/Methodology
Propidium iodide dye binding to DNA after cell membrane permeabilization. Analyzed on a flow cytometer
Reference Ranges
Interpretation reported by Pathologist
CPT
88182