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HelpTest Code F_10 Coagulation Factor X Activity Assay, Plasma
Ordering Guidance
Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information. For that reason, consider ordering a Coagulation Consultation.
Necessary Information
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Light-blue top (3.2% sodium citrate)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL Platelet-poor plasma
Collection Instructions:
1. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.
2. Within 4 hours of collection, centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.
3. Aliquot plasma into a separate plastic vial, leaving 0.25 mL in the bottom of the centrifuged vial.
4. Immediately freeze plasma (no longer than 4 hours after collection) at -20° C or, ideally, -40° C or below.
Additional Information:
1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. Each coagulation assay requested should have its own vial.
CMH COLLECTION: 2.7 mL in light blue vacutainer
Specimen Minimum Volume
Platelet-poor plasma: 0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma Na Cit | Frozen | 14 days |
Useful For
Diagnosing deficiency of coagulation factor X, congenital or acquired
Evaluating hemostatic function in liver disease
Investigation of prolonged prothrombin time or activated partial thromboplastin time
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysReporting Name
Coag Factor X Assay, PReference Values
Adults: 70-150%
Normal, full-term newborn infants or healthy premature infants may have decreased levels (≥15-20%), which may not reach adult levels for 180 or more days postnatal.*
*See Pediatric Hemostasis References section in Coagulation Guidelines for Specimen Handling and Processing
Method Name
Optical Clot-Based
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
85260