Antinuclear Antibody (ANA)
Collection Requirements
Source: blood
Container: Red gel
Volume: 1.5 mL in vacutainer
Turn Around Time
1-4 days
Batched testing performed daily Monday – Thursday
Availability
Routine
Algorithm
If ELISA screen is equivocal or positive then IFA will be done and titer reported
Lab Processing Instructions
Specimen: serum
Spin, separate, and refrigerate
Performing Laboratory
Adele Hall
Adele Hall Lab Section
Immunology
Instrumentation/Methodology
ELISA, IFA
Reference Ranges
ELISA
Negative < 20
Equivocal 20 - 60
Positive ≥60
IFA negative
CPT
86038
second 86038 if needed
third 86038 if needed