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Antinuclear Antibody (ANA)

Collection Requirements

Source: blood
Container: Red gel
Volume: 1.5 mL in vacutainer

Turn Around Time

1-4 days
Batched testing performed daily Monday – Thursday

Availability

Routine

Algorithm

If ELISA screen is equivocal or positive then IFA will be done and titer reported

Lab Processing Instructions

Specimen: serum
Spin, separate, and refrigerate

Performing Laboratory

Adele Hall

Adele Hall Lab Section

Immunology

Instrumentation/Methodology

ELISA, IFA

Reference Ranges

ELISA
Negative < 20
Equivocal 20 - 60
Positive ≥60

IFA negative

CPT

86038
second 86038 if needed
third 86038 if needed