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HelpAntinuclear Antibody Reflex
Collection Requirements
Source: blood
Container: Red gel
Volume: 1.5 mL in vacutainer
Turn Around Time
2-4 days ANA ELISA & IFA and titer if needed – batched testing performed Monday, Tuesday, and Thursday
AENA (Antibodies to Extractable Nuclear Antigen) is a Sendout
Availability
Routine
Algorithm
If ANA ELISA is >20 then ANA IFA will be done
If ANA IFA Titer is ≥160 then reflex AENA and dsDNA
Lab Processing Instructions
Specimen: serum
Spin, separate, and refrigerate
Performing Laboratory
Adele Hall
Adele Hall Lab Section
Immunology
Instrumentation/Methodology
ANA ELISA, IFA
DsDNA IFA
AENA Sendout
Reference Ranges
ANA ELISA
Negative < 20
Eguivocal 20-60
Positive > 60
ANA IFA negative
DsDNA negative
AENA see report
CPT
86038
additional 86038 if needed
86039 if needed
86255 if needed
86235 x6 if needed