Instrumentation/Methodology

Vitamin D BP is quantitated using a sandwich immunoassay using monoclonal antibodies , one as capture and other as tag antibody , conjugated with horse raddish peroxidase (HRP). The color is developed and read at 450 nm .The Optical density is directly proportional to the concentration of DBP. Monoclonal antibodies are capable recognizing all the fractions of Vitamin D BP.