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HelpTest Code AFPA Alpha-Fetoprotein, Amniotic Fluid
Necessary Information
The following information is required:
1. Estimated due date by ultrasound
2. Collection date
3. Gestational age must be between 13 and 24 weeks; 16 to 18 weeks preferred.
If not ordering electronically, provide information on Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information (T595) and send with specimen.
Specimen Required
Container/Tube: Amniotic fluid container
Specimen Volume: 0.75 mL
Collection Instructions: Do not centrifuge.
CMH COLLECTION: 1 mL in sterile cup
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Amniotic Fld | Refrigerated (preferred) | 7 days | |
| Ambient | 7 days |
Testing Algorithm
If alpha-fetoprotein is positive, then acetylcholinesterase will be performed at an additional charge.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ACHE_ | Acetylcholinesterase, AF | Yes | No |
Useful For
Screening for open neural tube defects or other fetal abnormalities
Follow-up testing for patients with elevated serum alpha-fetoprotein results or in conjunction with cytogenetic testing
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Day(s) Performed
Monday through Friday
Report Available
2 to 19 daysReporting Name
Alpha Fetoprotein, AFReference Values
< 2.0 multiples of median (MoM)
Method Name
AFPA: Immunoenzymatic Assay
ACHE_: Polyacrylamide Electrophoresis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82106
82013 (if appropriate)