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HelpPlatelet Aggregation with Secretion Studies
Collection Restrictions
Call Hematology before collection
Testing is limited to a maximum of 6 patients each day of testing
Can ONLY collect:
- Monday - Friday
- 7:00 a.m. - 2:00 p.m.; specimens MUST be received in the Lab BEFORE 2:00 p.m.
Collection Requirements
Source: blood
Containers: Sodium citrate light blue and EDTA lavender
Volume:
- Sodium citrate: Four 2.7 mL in vacutainers filled to the line
- EDTA: 1 mL in vacutainer or 250 uL in microtainer
- If a CBC or CBCD is also ordered the EDTA can be shared
Special Instructions
Send to the lab immediately
Must be tested within 3 hours of collection
Turn Around Time
24-72 hours
Availability
Routine
Includes
Aggregation: ADP, Arachidonic Acid, Collagen 5, Collagen 1, Ristocetin 1, and Ristocetin 0.25
Secretion: ADP, Arachidonic Acid, Collagen 5, Collagen 1, and Thrombin
Lab Processing Instructions
Specimen: whole blood
Room temperature
- Do not check for clots
Offsites: Send whole blood at room temperature with a STAT courier
Performing Laboratory
Adele Hall
Adele Hall Lab Section
Hematology
Instrumentation/Methodology
Whole blood impedance
Reference Ranges
Report includes maximum aggregation and secretion as well as pathologist interpretation
| Agonist | Aggregation (ohms) | Secretion (nM) |
|---|---|---|
| ADP, 10 uM | > 6 | >0.25 |
| Arachidonic Acid, 0.5 mM | > 9 | >0.50 |
| Collagen, 5 ug/mL | > 9 | >0.67 |
| Collagen, 1 ug/mL | > 8 | >0.40 |
| Ristocetin, 1 mg/mL | >5 | n/a |
| Ristocetin, 0.25 mg/mL | 0 | n/a |
| Thrombin, 1 Unit/mL | n/a | >0.62 |
CPT
85576 x7
82397 x5